Khaws cov phev

Qhov hom kev khaws cov phev (ejaculate), zoo li cryopreservation, tau ua rau hauv ntau tus cryomedicine. Nws suav nrog qhov sib ntxiv ntawm lub nruab nrab tshwj xeeb rau lub seminal fluid, thiab ntxiv kom khov nws nrog kev siv cov kua nitrogen vapors. Xav txog hom kev tiv thaiv kabmob cov kabmob txivneej hauv ntau yam thiab qhia rau koj seb cov phev puas tsim nyog rau dilution thiab ntxiv cia.

Ua li cas cryopreservation tau nce txog qhov uas yuav tau siv cov tshuaj fertilization?

Nrog rau kev yug menyuam, daim ntawv qog ntawm tus txiv neej qog qe yog sib xyaw nrog lub hlwb ntawm cov epidermis, yog li ntawd, muaj kev hloov pH ntawm qhov nruab nrab tshwm sim, uas ua rau kev puas tsuaj ntawm lub lipoprotein cover ntawm spermatozoa, i.e. rau lawv ua kom. Nyob hauv tus mob no, lub neej ntawm cov qoob noob qoob noob hnyav yog loj heev, uas tsis suav nrog lawv siv IVF. Tias yog vim li cas txoj kev siv cryopreservation.

Cov txheej txheem no ua rau lub neej txuag ntawm cov phev, thiab tso cai rau:

• txo cov kev ua kom lub cev muaj zog ntawm spermatozoa;
• muab lawv nrog lwm qhov chaw muaj zog;
• tiv thaiv cov kab mob ntawm cov kab mob ntawm cov kab mob ntawm kev puas tsuaj;
• tiv thaiv txuam nrog cov khoom siv tshuaj lom metabolic;
• blocks cov luam ntawm microorganisms nyob rau hauv lub ejaculate.

Dab tsi siv nruab nrab yog siv los khaws cov phev?

Yuav kom yooj yim, txoj kev sib txuas lus ntawm crushing lub ejaculate, tsim nyog rau kev sib cais ntawm cov kab mob ntawm nws, ua ntej-dilute cov phev. Siv cov tshuaj reagents tshwj xeeb.

Txog rau hnub tim, nws yog txoj kev cai siv hluavtaws tawm rau cov phev cia, txhua yam uas yuav tsum tau muaj kev tshwj xeeb. Feem ntau, hom cheeb tsam no muaj ob peb yam, feem ntau peb. Yog li, nyob rau hauv lawv cov qauv nws yog ib qho ua tau kom faib cov suab thaj, feem ntau thiab siv cov suab thaj thiab lactose, sodium citrate.

Yog hais tias peb tham txog cov tshuaj chemical uas siv tau los ua kev tshaj tawm rau kev txuag cov phev, ces ntawm cov no hu ua Tris-buffer, Trilon B, EDTA, Spermosan PPK.

Nyob rau hauv dab tsi mob yog ejaculate cia?

Cov cuab yeej ntawm kev khov thiab cov phev cia yuav tsum ua raws li kev tswj hwm txias, thiab siv cov cuab yeej tshwj xeeb. Hauv qhov no, chav kuaj chav kuaj mob yuav tsum tau nruab nrog cov cuab yeej cua kom tsis txhob muaj cua ntsawj ntshab.

Ua ntej lub ejaculate tau sau, tag nrho cov khoom tsim nyog rau cryopreservation, tshwj xeeb tshaj yog txhua yam ntawm cov khoom siv, kawm tiav thooj voos, cov kav dej, cov ntxaij lim dej yog cov sterilized hauv lub txee tshwj xeeb, ntawm qhov kub ntawm 130-150 degrees. Ua ntej ua tus txheej txheem coj mus kuaj, lawv muab tso rau hauv ib lub ntsuas cua, tshwj xeeb uas tswj qhov kub ntawm 37 degrees.

Thaum tus txiv neej cov phev txiv neej coj mus lawm, nws muab tso rau hauv lub hwj sib phim. Qhov cia cov xim ntawm cov phev yuav tsum tsis tu ncua. Cov txheej txheem txias yog nqa tawm hauv theem 2.

Nyob rau ntawm thawj ntawm lawv, lub ejaculate yog thawj muab tso rau hauv ib chav tsev txias, nyob rau hauv uas kub yog maj txo. Raws li txoj cai, nws tus nqi yog -35 degrees. Tom qab qhov no, sib zog nqus khov yog nqa tawm, muab lub hwj iav tshwj xeeb nrog cov phev ua kua nitrogen. Hauv qhov no, lub txee lub neej ntawm cov phev tuaj yeem ncav ntau ntau xyoo.

Siv ejaculate, cryopreserved ntxov, lub nkoj nrog nws muab tso rau hauv dej sov, qhov twg qeeb yaj yuav siv qhov chaw. Tom qab ntawd, cov siv cryoprotectants raug tshem tawm, los ntawm kev rov ua dua ntawm cov phev ntawm cov centrifuge. Tom qab ntawd, cov kua nruab nrab ntawm nws tus kheej yog hloov nrog ib tus neeg nruab nrab rau hauv uas cov kab mob spermatozoa tom qab muab tso rau.